Micro-injection into zebrafish blastocyst. If you're new to zebrafish injections, read this published protocol and watch the associated video.
DNA and RNA work
PCR master mix recipie
Generation of single guide (sg) RNAs
In situ hybridization
Whole mount in situ hybridization (one color) and checklist
Whole mount in situ hybridization (two color) and checklist
Fluorescence in situ hybridization