Preparing oligo stocks
Generation of single guide RNAs by assembling oligos and in vitro RNA synthesis
Synthesis of gRNAs in multiplex
Zebrafish injections. If you are new to injections, watch the video associated with this manuscript to get an idea of the process.
Generation of DIG-labelled riboprobes from synthesized templates
RNA extraction from zebrafish embryos (18-32 hpf)