lab Protocols

 

Zebrafish procedures

Micro-injection into zebrafish blastocyst. If you're new to zebrafish injections, read this published protocol and watch the associated video.

DNA and RNA work

PCR master mix recipie

Generation of single guide (sg) RNAs

In situ hybridization

Whole mount in situ hybridization (one color) and checklist

Whole mount in situ hybridization (two color) and checklist

Fluorescence in situ hybridization